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Mol Cells. 2019 Sep 4. doi: 10.14348/molcells.2019.0173. [Epub ahead of print]

Arabidopsis Raf-Like Kinase Raf10 Is a Regulatory Component of Core ABA Signaling.

Author information

1
Department of Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea.
2
Department of New Biology, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu 42988, Korea.
3
Department of Molecular Biology, Sejong University, Seoul 05006, Korea.
#
Contributed equally

Abstract

Abscisic acid (ABA) is a phytohormone essential for seed development and seedling growth under unfavorable environmental conditions. The signaling pathway leading to ABA response has been established, but relatively little is known about the functional regulation of the constituent signaling components. Here, we present several lines of evidence that Arabidopsis Raf-like kinase Raf10 modulates the core ABA signaling downstream of signal perception step. In particular, Raf10 phosphorylates subclass III SnRK2s (SnRK2.2, SnRK2.3, and SnRK2.6), which are key positive regulators, and our study focused on SnRK2.3 indicates that Raf10 enhances its kinase activity and may facilitate its release from negative regulators. Raf10 also phosphorylates transcription factors (ABI5, ABF2, and ABI3) critical for ABAregulted gene expression. Furthermore, Raf10 was found to be essential for the in vivo functions of SnRK2s and ABI5. Collectively, our data demonstrate that Raf10 is a novel regulatory component of core ABA signaling.

KEYWORDS:

ABI5; Raf10; SnRK2s; abscisic acid; phytohormone; signaling

PMID:
31480825
DOI:
10.14348/molcells.2019.0173
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