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Curr Protoc Stem Cell Biol. 2019 Sep;50(1):e95. doi: 10.1002/cpsc.95.

Differentiation of Retinal Organoids from Human Pluripotent Stem Cells.

Author information

1
Institute of Genetic Medicine, International Centre for Life, Newcastle University, Newcastle upon Tyne, United Kingdom.
2
Newcells Biotech Ltd, Biomedicine West Wing, International Centre for Life, Newcastle upon Tyne, United Kingdom.

Abstract

This unit describes a protocol for generating retinal organoids that contain all major retinal cell types and are responsive to light from human pluripotent stem cells (hPSCs). hPSCs are differentiated in 96-well plates to allow large-scale production of organoids that could be used for multiple applications, including study of human retinal development, disease modeling, and compound screening. The differentiation approach is based on the knowledge that insulin-like growth factor 1 signaling together with retinoic acid and triiodothyronine is important for retinal development. After 22 weeks in culture, the organoids form a thick layer of neuroepithelium containing photoreceptors and bipolar, horizontal, amacrine, Müller, and retinal ganglion cells. Differentiation progress can be tracked by morphological observations and protein localization, as detected with immunocytochemistry.

KEYWORDS:

differentiation; induced pluripotent stem cells; organoids; retina

PMID:
31479596
DOI:
10.1002/cpsc.95

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