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PLoS One. 2019 Sep 3;14(9):e0221900. doi: 10.1371/journal.pone.0221900. eCollection 2019.

Analysis of potential roles of combinatorial microRNA regulation in occurrence of valvular heart disease with atrial fibrillation based on computational evidences.

Wang G1,2,3, Rao N1,2,3,4, Liu D1,2,3, Jiang H1,2,3, Liu K5, Yang F1,2,3, Chen Y2, Huang K5.

Author information

1
Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
2
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
3
Key Laboratory for NeuroInformation of the Ministry of Education, University of Electronic Science and Technology of China, Chengdu, China.
4
Institute of Electronic and Information Engineering of UESTC in Guangdong, Dongguan, China.
5
Subsidiary Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Abstract

BACKGROUND:

Atrial fibrillation (AF) is the most common arrhythmia. Patients with valvular heart disease (VHD) frequently have AF. Growing evidence demonstrates that a specifically altered pattern of microRNA (miRNA) expression is related to valvular heart disease with atrial fibrillation (AF-VHD) processes. However, the combinatorial regulation by multiple miRNAs in inducing AF-VHD remains largely unknown.

METHODS:

The work identified AF-VHD-specific miRNAs and their combinations through mapping miRNA expression profile into differential co-expression network. The expressions of some dysregulated miRNAs were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The regulations of signaling pathways by the combinatorial miRNAs were predicted by enrichment analysis tools.

RESULTS:

Thirty-two differentially expressed (DE) miRNAs were identified to be AF-VHD-specific, some of which were new findings. These miRNAs interacted to form 5 combinations. qRT-PCR confirmed the different expression of several identified miRNAs, which illustrated the reliability and biomarker potentials of 32 dysregulation miRNAs. The biological characteristics of combinatorial miRNAs related to AF-VHD were highlighted. Twelve signaling pathways regulated by combinatorial miRNAs were predicted to be possibly associated with AF-VHD.

CONCLUSIONS:

The AF-VHD-related signaling pathways regulated by combinatorial miRNAs may play an important role in the occurrence of AF-VHD. The work brings new insights into biomarkers and miRNA combination regulation mechanism in AF-VHD as well as further biological experiments.

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