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Stem Cell Res. 2019 Aug 22;40:101546. doi: 10.1016/j.scr.2019.101546. [Epub ahead of print]

Generation of a human induced pluripotent stem cell line, IMSUTi002-A-1, harboring the leukemia-specific fusion gene ETV6-RUNX1.

Author information

1
Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI), Chiba, Japan. Electronic address: takamasa@criepi.denken.or.jp.
2
The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Abstract

Reprograming human primary cancer cells remains technically challenging, and leukemia-specific human induced pluripotent stem cell (hiPSC) lines have not yet been generated from tissues of patients with acute leukemia (Muñoz-López et al., 2016). Here, we developed an hiPSC line with a doxycycline-inducible expression system with the fusion gene ETV6-RUNX1 integrated into the genome. This is the most common genetic aberration found in childhood leukemia. This hiPSC line will be a useful model for research on childhood leukemia pathology and risk assessment.

PMID:
31476588
DOI:
10.1016/j.scr.2019.101546
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