Format

Send to

Choose Destination
Plast Reconstr Surg. 2019 Aug 27. doi: 10.1097/PRS.0000000000006266. [Epub ahead of print]

Involvement of PDGF-BB and IGF-1 in activation of human Schwann cells by platelet-rich plasma.

Author information

1
Department of Plastic and Reconstructive Surgery, Graduate School of Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
2
Departments of Immunology, Graduate School of Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
3
Department of Plastic Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
4
Dental Medicine, Graduate School of Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

Abstract

BACKGROUND:

Platelet-rich plasma (PRP) contains high concentrations of growth factors that stimulate proliferation and migration of various cell types. Earlier experiments demonstrated that local PRP administration activates Schwann cells (SCs) to improve axonal regeneration at a transected peripheral nerve lesion. However, the optimal concentration of human PRP for activation of human SCs (hSCs) has not been determined, while mechanisms by which PRP activates SCs remain to be clarified.

METHODS:

hSCs were cultured with various concentrations of PRP in 5% FBS/DMEM. Cell viability, microchemotaxis, flowcytometry, and quantitative RT-PCR assays were performed to assess proliferation, migration, cell cycle, and neurotrophic factor expression of the hSCs, respectively. hSCs were co-cultured with neuronal cells to assess their capacity to induce neurite extension. Neutralizing antibodies for platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-1 (IGF-1) were added to the culture to estimate contribution of these cytokines to the hSC stimulation by PRP.

RESULTS:

An addition of PRP at 5% strongly elevated proliferation, migration, and neurotrophic factor production of hSCs. Both PDGF-BB and IGF-1 may be involved in mitogenic effect of PRP on hSCs, while PDGF-BB may also play an important role in the migration-inducing effect of PRP. Neutralization of both PDGF-BB and IGF-1 cancelled promoting effect of PRP on neurite-inducing activity of hSCs.

CONCLUSIONS:

The present findings may suggest the optimal concentration of PRP for hSC stimulation as well as potential mechanisms underlying the activation of hSCs by PRP, which may be quite useful for the PRP therapy for peripheral nerve regeneration.

Supplemental Content

Full text links

Icon for Wolters Kluwer
Loading ...
Support Center