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Stem Cell Res. 2019 Aug 9;40:101534. doi: 10.1016/j.scr.2019.101534. [Epub ahead of print]

Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts.

Author information

1
Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, GKVK post, Bellary Road, Bengaluru, Karnataka 560065, India.
2
Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, GKVK post, Bellary Road, Bengaluru, Karnataka 560065, India. Electronic address: shagufta.parveen@manipal.edu.

Abstract

Circumcision is a common surgical procedure usually performed on neonates worldwide. Skin removed after circumcision is abundant and often discarded. Neonatal foreskin fibroblasts (NFFs) can be easily isolated from it for application in biomedical research including induced pluripotent stem cell (iPSC) generation. In this study NFFs were reprogrammed to iPSCs using non-integrating episomal reprogramming plasmids. The iPSCs generated express pluripotency markers. They form embryoid bodies and differentiate in vitro into cells of endoderm, mesoderm and ectoderm. They have a normal male karyotype, are genetically identical to the parent fibroblasts and are transgene free.

PMID:
31454679
DOI:
10.1016/j.scr.2019.101534
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