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Methods Mol Biol. 2020;2052:335-350. doi: 10.1007/978-1-4939-9748-0_19.

In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds.

Author information

1
Haskins Laboratories, Pace University, New York, NY, USA. nyarlett@pace.edu.
2
The Department of Chemistry and Physical Chemistry, Pace University, New York, NY, USA. nyarlett@pace.edu.
3
Haskins Laboratories, Pace University, New York, NY, USA.
4
Division of Allergy and Infectious Disease, Department of Medicine, The Center for Emerging and Re-emerging Infectious Diseases, University of Washington, Seattle, WA, USA.

Abstract

Hollow fiber technology is a powerful tool for the culture of difficult-to-grow cells. Cryptosporidium parvum has a multistage sexual and asexual life cycle that has proved difficult to culture by conventional in vitro culture methods. Here, we describe a method utilizing a hollow fiber bioreactor for the continuous in vitro growth of C. parvum that produces sexual and asexual stages. The method enables the evaluation of potential therapeutic compounds under conditions that mirror the dynamic conditions found in the gut facilitating preliminary pharmacokinetic and pharmacodynamic data to be obtained.

KEYWORDS:

Cryptosporidium parvum; Hollow fiber bioreactor; In vitro culture; Pharmacodynamics; Pharmacokinetic

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