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Methods Mol Biol. 2020;2052:283-302. doi: 10.1007/978-1-4939-9748-0_16.

Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water.

Author information

1
Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. JLMurphy@cdc.gov.
2
Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Abstract

This chapter provides a detailed protocol to assess disinfection efficacy of chlorine against Cryptosporidium oocysts including the core chlorine disinfection assay, the in vitro cell culture infectivity assay, and microscopy analysis and data interpretation.

KEYWORDS:

Cell culture infectivity; Chlorine; Cryptosporidium; Disinfection; In vitro culture; Oocysts

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