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Methods Mol Biol. 2020;2052:23-41. doi: 10.1007/978-1-4939-9748-0_3.

Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration.

Author information

1
Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Diseases Control and Prevention, Atlanta, GA, USA. AKahler@cdc.gov.
2
Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Diseases Control and Prevention, Atlanta, GA, USA.

Abstract

The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.

KEYWORDS:

Cryptosporidium; Dead-end ultrafiltration; IMS; Microscopy; Real-time PCR; Water

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