Format

Send to

Choose Destination
Nat Methods. 2019 Sep;16(9):925-931. doi: 10.1038/s41592-019-0536-2. Epub 2019 Aug 26.

Transmission-mode MALDI-2 mass spectrometry imaging of cells and tissues at subcellular resolution.

Author information

1
Institute for Hygiene, University of Münster, Münster, Germany.
2
Interdisciplinary Center for Clinical Research, University of Münster, Münster, Germany.
3
Spectroglyph LLC, Kennewick, WA, USA.
4
Institute for Hygiene, University of Münster, Münster, Germany. klaus.dreisewerd@uni-muenster.de.
5
Interdisciplinary Center for Clinical Research, University of Münster, Münster, Germany. klaus.dreisewerd@uni-muenster.de.

Abstract

Matrix-assisted laser desorption-ionization mass spectrometry imaging in transmission-mode geometry (t-MALDI-MSI) can provide molecular information with a pixel size of 1 µm and smaller, which makes this label-free method highly interesting for characterizing the chemical composition of tissues and cells on a (sub)cellular level. However, a major hindrance for wider use of the technology is the reduced ion abundance at small pixel sizes. Here we mitigate this problem by use of laser-induced post-ionization (MALDI-2) and by adapting a t-MALDI-2 ion source to an Orbitrap mass analyzer. We demonstrate the crucial sensitivity and accuracy boosts that are achieved with this combination by visualizing the distribution of numerous phospho- and glycolipids in mouse cerebellum and kidney slices, and in cultured Vero B4 cells. With brain tissue, a pixel size of 600 nm was achieved. Our method could constitute a valuable new tool for research in cell biology and biomedicine.

PMID:
31451764
DOI:
10.1038/s41592-019-0536-2
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center