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Stem Cell Res. 2019 Jul 31;40:101520. doi: 10.1016/j.scr.2019.101520. [Epub ahead of print]

Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9.

Author information

1
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
2
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea. Electronic address: kjhcorea@korea.kr.

Abstract

Toll Like Receptor (TLR) family plays an important role in the activation of innate immunity against pathogens. TLR7 mediates the recognition of single-stranded RNA viruses, such as human immunodeficiency virus, hepatitis C virus, and influenza virus in endosomes. Here, we generated a TLR7 homozygous knockout human induced pluripotent cell (hiPSC) line, hiPSC-TLR7KO-A59, using the CRISPR/Cas9 genome editing method. The hiPSC-TLR7KO-A59 line maintains normal morphology, pluripotency, and differentiation capacity into three germ layers.

PMID:
31445394
DOI:
10.1016/j.scr.2019.101520
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