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Stem Cell Res. 2019 Aug 14;40:101530. doi: 10.1016/j.scr.2019.101530. [Epub ahead of print]

Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming.

Author information

1
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
2
University of Sydney ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, Australia.
3
Macquarie University Centre for Motor Neuron Disease Research, Macquarie University Faculty of Medicine and Health Sciences, NSW 2109, Australia.
4
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address: lezanne@uow.edu.au.

Abstract

Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

PMID:
31445393
DOI:
10.1016/j.scr.2019.101530
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