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Heart Vessels. 2019 Aug 23. doi: 10.1007/s00380-019-01486-y. [Epub ahead of print]

Clinically feasible method for assessing leukocyte rheology in whole blood.

Author information

1
Department of Cardiac and Vascular Surgery, Dokkyo Medical University Nikko Medical Center, Nikko, Tochigi, Japan.
2
Department of Cardiac and Vascular Surgery, Dokkyo Medical University, Mibu, Tochigi, Japan.
3
Kikuchi Microtechnology Institute, Ryugasaki, Ibaraki, Japan.
4
Department of Clinical Laboratory, Dokkyo Medical University Nikko Medical Center, Nikko, Tochigi, Japan.
5
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
6
Department of Diabetes and Endocrinology, Dokkyo Medical University Nikko Medical Center, Nikko, Tochigi, Japan.
7
Department of Rehabilitation, Dokkyo Medical University Nikko Medical Center, Nikko, Tochigi, Japan.
8
Department of Cardiovascular Medicine and Nephrology, Dokkyo Medical University Nikko Medical Center, 632 Takatoku, Nikko, 321-2593, Tochigi, Japan.
9
Department of Cardiology, Dokkyo Medical University Nikko Medical Center, Nikko, Tochigi, Japan.
10
Department of Cardiovascular Medicine and Nephrology, Dokkyo Medical University Nikko Medical Center, 632 Takatoku, Nikko, 321-2593, Tochigi, Japan. tyasu@dokkyomed.ac.jp.

Abstract

This study reports a novel method for assessment of leukocyte rheological activation with a new designed microchannel array chip to mimic the human microvascular network for microchannel array flow analysis (MCFAN). Study subjects were 79 healthy volunteers and 42 patients with type 2 diabetes mellitus (DM) and 36 patients with acute coronary syndrome (ACS). Using the anticoagulants heparin and ethylene-diamine-tetraacetic acid (EDTA)-2Na which inhibits platelets and leukocytes by chelating Ca2+, we were able to quantify leukocyte rheological activation by the subtraction of passage time of blood treated with both heparin and EDTA-2Na from that of blood treated with heparin only. We confirmed that passage times of whole blood with heparin + EDTA-2Na were always shorter than those of whole blood with only heparin in healthy subjects and patients with DM or ACS under suction pressures of - 30 cmH2O. There was a significant correlation between delta whole blood passage time {(heparin tube) - (EDTA-2Na + heparin)} and serum levels of myeloperoxidase and adhesive leukocyte number, respectively, even in blood from patients with DM or ACS, who suffered from inflammation. In conclusion we have developed a clinically feasible method for assessing leukocyte rheological activation in whole blood in ex vivo.

KEYWORDS:

Acute coronary syndrome; Diabetes mellitus; Leukocyte; Microcirculation; Rheology

PMID:
31444563
DOI:
10.1007/s00380-019-01486-y

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