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Gastroenterology. 2019 Aug 20. pii: S0016-5085(19)41233-X. doi: 10.1053/j.gastro.2019.08.019. [Epub ahead of print]

Activation of Hedgehog Signaling Promotes Development of Mouse and Human Enteric Neural Crest Cells, Based on single-cell Transcriptome Analyses.

Author information

1
Department of Surgery, Li Ka Shing Faculty of Medicine; Dr. Li Dak Sum Research Centre, The University of Hong Kong, Pokfulam, Hong Kong.
2
Department of Surgery, Li Ka Shing Faculty of Medicine.
3
Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USA.
4
CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, China.
5
Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USA; Inserm UMR 1235 - TENS, Inserm, University of Nantes, 44035 Nantes, France.
6
Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, and Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
7
Department of Surgery, Li Ka Shing Faculty of Medicine. Electronic address: engan@hku.hk.

Abstract

BACKGROUND & AIMS:

It has been a challenge to develop fully functioning cells from human pluripotent stem cells (hPSCs). We investigated how activation of hedgehog signaling regulates derivation of enteric neural crest (NC) cells from hPSCs.

METHODS:

We analyzed transcriptomes of mouse and hPSC-derived enteric NCs using single-cell RNA sequencing (scRNA-seq) to identify changes in expression associated with lineage differentiation. Intestine tissues were collected from Tg(GBS-GFP), Sufuf/f; Wnt1-cre, Ptch1+/- and Gli3Δ699/Δ699 mice and analyzed by flow cytometry and immunofluorescence for levels of mRNAs encoding factors in the hedgehog signaling pathway during differentiation of enteric NCs. Human NC cells (HNK-1+ p75NTR+) were derived from IMR90 and UE02302 hPSC lines. hPSC were incubated with hedgehog agonists (SAG) and antagonists (cyclopamine) and analyzed for differentiation. hPSC-based innervated colonic organoids were derived from these hPSC lines and analyzed by immunofluorescence and neuromuscular coupling assay for expression of neuronal subtype markers and for assessing the functional maturity of the hPSC-derived neurons, respectively.

RESULTS:

scRNA-seq analysis revealed that neural fate acquisition by human and mouse enteric NCs requires reduced expression of NC- and cell cycle- specific genes and upregulation of neuronal- or glial-lineage specific genes. Activation of the hedgehog pathway was associated with progression of mouse enteric NCs to the more mature state along the neuronal and glial lineage differentiation trajectories. Activation of the hedgehog pathway promoted development of cultured hPSC into NCs of greater neurogenic potential by activating expression of genes in the neurogenic lineage. The hedgehog agonist increased differentiation of hPSCs into cells of the neuronal lineage by upregulating expression of GLI2 target genes, including INSM1, NHLH1, and various bHLH family members. The hedgehog agonist increased expression of late neuronal markers and neuronal activities in hPSC-derived neurons.

CONCLUSIONS:

In enteric NCs from humans and mice, activation of hedgehog signaling promotes differentiation into neurons by promoting cell-state transition, expression of genes in the neurogenic lineage, and functional maturity of enteric neurons.

KEYWORDS:

Enteric nervous system; Hirschsprung disease; disease model; regenerative medicine

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