Format

Send to

Choose Destination
Emerg Infect Dis. 2019 Sep;25(9):1730-1735. doi: 10.3201/eid2509.190205.

Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity.

Abstract

Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney-infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.

KEYWORDS:

Ct cutoff; Cycle threshold; Hong Kong; enteric infections; enteroids; human norovirus; infectivity; rRT-PCR; real-time reverse transcription PCR; viruses

PMID:
31441758
PMCID:
PMC6711227
DOI:
10.3201/eid2509.190205
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for CDC-NCEZID Icon for PubMed Central
Loading ...
Support Center