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Science. 2019 Aug 23;365(6455). pii: eaaw2619. doi: 10.1126/science.aaw2619.

The Malaria Cell Atlas: Single parasite transcriptomes across the complete Plasmodium life cycle.

Author information

1
Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge CB10 1SA, UK.
2
Danish Institute of Advanced Study (D-IAS), Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
3
International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.
4
Wits Research Institute for Malaria, MRC Collaborating Centre for Multi-disciplinary Research on Malaria, School of Pathology, Faculty of Health Sciences, University of the Witswatersrand, Johannesburg, South Africa.
5
Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
6
MRC-University of Glasgow Centre for Virus Research, Glasgow, UK.
7
Laboratory for Molecular Infection Medicine Sweden, Department of Molecular Biology, Umeå University, Umeå, Sweden.
8
MIVEGEC, IRD, CNRS, University of Montpellier, Montpellier, France.
9
Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge CB10 1SA, UK. mara@sanger.ac.uk.

Abstract

Malaria parasites adopt a remarkable variety of morphological life stages as they transition through multiple mammalian host and mosquito vector environments. We profiled the single-cell transcriptomes of thousands of individual parasites, deriving the first high-resolution transcriptional atlas of the entire Plasmodium berghei life cycle. We then used our atlas to precisely define developmental stages of single cells from three different human malaria parasite species, including parasites isolated directly from infected individuals. The Malaria Cell Atlas provides both a comprehensive view of gene usage in a eukaryotic parasite and an open-access reference dataset for the study of malaria parasites.

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PMID:
31439762
DOI:
10.1126/science.aaw2619

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