Preparation of Cholera Toxin Subunit B Functionalized Nanoparticles for Targeted Therapy of Glioblastoma

Juan Guan; Jun Qian; Changyou Zhan

doi:10.1007/978-1-4939-9798-5_10

Preparation of Cholera Toxin Subunit B Functionalized Nanoparticles for Targeted Therapy of Glioblastoma

Methods Mol Biol. 2020:2059:207-212. doi: 10.1007/978-1-4939-9798-5_10.

Authors

Juan Guan^{1

2

3

4}, Jun Qian^{3

4}, Changyou Zhan^{5

6

7}

Affiliations

¹ School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
² State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, People's Republic of China.
³ School of Pharmacy, Fudan University, Shanghai, People's Republic of China.
⁴ Key Laboratory of Smart Drug Delivery, Fudan University, Ministry of Education, Shanghai, People's Republic of China.
⁵ School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China. cyzhan@fudan.edu.cn.
⁶ State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, People's Republic of China. cyzhan@fudan.edu.cn.
⁷ Key Laboratory of Smart Drug Delivery, Fudan University, Ministry of Education, Shanghai, People's Republic of China. cyzhan@fudan.edu.cn.

PMID: 31435923
DOI: 10.1007/978-1-4939-9798-5_10

Abstract

Cholera toxin subunit B (CTB) is the nontoxic moiety of cholera toxin. It can target the glycosphingolipid GM1 expressed in the blood-brain barrier (BBB), neovasculature, and glioblastoma cells. Thus, CTB has been utilized as a multifunctional molecule for targeted therapy of glioblastoma. Here, we describe a detailed method for preparation of CTB functionalized paclitaxel (PTX)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles. This unique modification can guide nanoparticles across the BBB and target glioblastoma cells. The characterization of nanoparticles such as size, zeta potential, morphology, drug loading, and encapsulation efficiency is shown in this chapter.

Keywords: Blood–brain barrier; Chemotherapeutic agents; Cholera toxin; Functionalized nanoparticles; Glioblastoma; Targeted delivery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents, Phytogenic / administration & dosage*
Antineoplastic Agents, Phytogenic / therapeutic use
Blood-Brain Barrier / metabolism*
Brain Neoplasms / drug therapy*
Brain Neoplasms / metabolism
Cholera Toxin / genetics
Cholera Toxin / metabolism*
Drug Carriers / chemistry*
Glioblastoma / drug therapy*
Glioblastoma / metabolism
Glycosphingolipids / metabolism
Microscopy, Electron, Transmission
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Neovascularization, Pathologic / drug therapy
Neovascularization, Pathologic / metabolism
Paclitaxel / administration & dosage*
Paclitaxel / therapeutic use
Particle Size
Polylactic Acid-Polyglycolic Acid Copolymer / chemistry

Substances

Antineoplastic Agents, Phytogenic
Drug Carriers
Glycosphingolipids
Polylactic Acid-Polyglycolic Acid Copolymer
Cholera Toxin
Paclitaxel