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Oxid Med Cell Longev. 2019 Jul 24;2019:3765643. doi: 10.1155/2019/3765643. eCollection 2019.

Effects of Individualized Treadmill Endurance Training on Oxidative Stress in Skeletal Muscles of Transgenic Sickle Mice.

Author information

1
Interuniversity Laboratory of Human Movement Biology EA7424, Vascular Biology and Red Blood Cell Team, University Claude Bernard Lyon 1, Villeurbanne, France.
2
Laboratory of Excellence "GR-Ex", Paris, France.
3
Division of Hematology/Oncology, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
4
Division of Endocrinology and Metabolism, University of Pittsburgh, Pittsburgh, PA 15261, USA.
5
Department of Biology, Chatham University, Pittsburgh, PA 15232, USA.
6
Center for Translational and International Hematology, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.
7
Interuniversity Laboratory of Human Movement Biology EA7424, Vascular Biology and Red Blood Cell Team, University Savoie Mont Blanc, Chambéry, France.
8
Institut Universitaire de France, Paris, France.

Abstract

Oxidative stress is a key feature in the pathophysiology of sickle cell disease. Endurance training has been shown to reduce oxidative stress in the heart and the liver of sickle mice. However, the effects of endurance training on skeletal muscles, which are major producers of reactive oxygen species during exercise, are currently unknown. The aim of this study was to evaluate the effect of sickle genotype on prooxidant/antioxidant response to individualized endurance training in skeletal muscles of sickle mice. Healthy and homozygous Townes sickle mice were divided into trained or sedentary groups. Maximal aerobic speed and V̇O2 peak were determined using an incremental test on a treadmill. Trained mice ran at 40% to 60% of maximal aerobic speed, 1 h/day, 5 days/week for 8 weeks. Oxidative stress markers, prooxidant/antioxidant response, and citrate synthase enzyme activities were assessed in the gastrocnemius, in the plantaris, and in the soleus muscles. Maximal aerobic speed and V̇O2 peak were significantly reduced in sickle compared to healthy mice (-57% and -17%; p < 0.001). NADPH oxidase, superoxide dismutase, and catalase activities significantly increased after training in the gastrocnemius of sickle mice only. A similar trend was observed for citrate synthase activity in sickle mice (p = 0.06). In this study, we showed an adaptive response to individualized endurance training on the prooxidant/antioxidant balance in the gastrocnemius, but neither in the plantaris nor in the soleus of trained sickle mice, suggesting an effect of sickle genotype on skeletal muscle response to endurance treadmill training.

PMID:
31428225
PMCID:
PMC6681588
DOI:
10.1155/2019/3765643
[Indexed for MEDLINE]
Free PMC Article

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