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Sci Rep. 2019 Aug 19;9(1):12046. doi: 10.1038/s41598-019-48603-5.

Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera).

Author information

1
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan.
2
St. Cousair Co., Ltd., 1260 Imogawa, Kami-minochi, Iizuna, Nagano, 389-1201, Japan.
3
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan.
4
Graduate School of Science and Technology, Department of Biomedical Engineering, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan.
5
Graduate School of Science and Technology, Department of Agriculture, Division of Food Science and Biotechnology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan.
6
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan. makabeh@shinshu-u.ac.jp.
7
Graduate School of Science and Technology, Department of Agriculture, Division of Food Science and Biotechnology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan. makabeh@shinshu-u.ac.jp.
8
Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan. makabeh@shinshu-u.ac.jp.
9
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan. hfujii@shinshu-u.ac.jp.
10
Graduate School of Science and Technology, Department of Biomedical Engineering, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan. hfujii@shinshu-u.ac.jp.
11
Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan. hfujii@shinshu-u.ac.jp.

Abstract

Novel proanthocyanidin fractions from grape stem extracts were purified using Amberlite XAD-1180N, Sephadex-LH-20, Toyopearl HW40F and reverse phase high-performance liquid chromatography. Two key compounds were estimated as epigallocatechin-(epicatechin)7 gallate using electron-spray ionization time-of-flight mass spectrometry. Epigallocatechin-(epicatechin)7 gallate (compound 1) showed significant anti-cancer activity in PC-3 prostate cancer cells. In particular, compound 1 suppressed the gene expression of fatty acid-binding protein 5 (FABP5), which is involved in promoting cell proliferation and metastasis in prostate cancer cells.

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