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Gigascience. 2019 Aug 1;8(8). pii: giz089. doi: 10.1093/gigascience/giz089.

A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata.

Author information

1
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health; WHO Collaborating Center for Tropical Diseases; Chinese Center for Tropical Diseases Research, Shanghai 200025, P. R. China.
2
Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan, Shanghai 200025, P. R. China.
3
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, P. R. China.
4
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266237, P. R. China.
5
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

Abstract

BACKGROUND:

Triatoma rubrofasciata is a widespread pathogen vector for Chagas disease, an illness that affects approximately 7 million people worldwide. Despite its importance to human health, its evolutionary origin has not been conclusively determined. A reference genome for T. rubrofasciata is not yet available.

FINDING:

We have sequenced the genome of a female individual with T. rubrofasciatausing a single molecular DNA sequencing technology (i.e., PacBio Sequel platform) and have successfully reconstructed a whole-genome (680-Mb) assembly that covers 90% of the nuclear genome (757 Mb). Through Hi-C analysis, we have reconstructed full-length chromosomes of this female individual that has 13 unique chromosomes (2n = 24 = 22 + X1 + X2) with a contig N50 of 2.72 Mb and a scaffold N50 of 50.7 Mb. This genome has achieved a high base-level accuracy of 99.99%. This platinum-grade genome assembly has 12,691 annotated protein-coding genes. More than 95.1% of BUSCO genes were single-copy completed, indicating a high level of completeness of the genome.

CONCLUSION:

The platinum-grade genome assembly and its annotation provide valuable information for future in-depth comparative genomics studies, including sexual determination analysis in T. rubrofasciata and the pathogenesis of Chagas disease.

KEYWORDS:

Triatoma rubrofasciata ; Hi-C; Iso-Seq; PacBio Sequel platform; RNA-Seq; chromosomal-level assembly; comparative genomics

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