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Indian J Med Microbiol. 2019 Jan-Mar;37(1):105-108. doi: 10.4103/ijmm.IJMM_18_146.

Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India.

Author information

1
Department of Microbiology, All Institute of Medical Sciences, New Delhi, India.
2
Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India.
3
Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
4
Centre for Community Medicine, All Institute of Medical Sciences, New Delhi, India.
5
Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
6
Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Abstract

Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.

KEYWORDS:

Micro-fluidic card; sensitivity; specificity; viral pathogen diagnosis

PMID:
31424019
DOI:
10.4103/ijmm.IJMM_18_146
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