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Indian J Med Microbiol. 2019 Jan-Mar;37(1):54-59. doi: 10.4103/ijmm.IJMM_18_409.

Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus.

Author information

1
Department of Microbiology, Diabetes and Metabolism, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
2
Department of Endocrinology, Diabetes and Metabolism, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

Abstract

Background:

Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens.

Aim and Objectives:

A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples.

Materials and Methods:

DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples.

Results:

P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914).

Conclusion:

Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status.

KEYWORDS:

Glycaemic control; Porphyromonas gingivalis; polymerase chain reaction; saliva

PMID:
31424011
DOI:
10.4103/ijmm.IJMM_18_409
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