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Indian J Med Microbiol. 2019 Jan-Mar;37(1):24-28. doi: 10.4103/ijmm.IJMM_18_264.

Institutional outbreak of varicella in a child welfare institute in Chandigarh, North India.

Author information

1
Department of Virology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
2
Department of Paediatrics, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.

Abstract

Introduction:

Varicella outbreaks are known to occur in developing nations as vaccine coverage is still low.

Material and Methods:

In the present study, an institutional outbreak from Chandigarh, India, is reported wherein the utility of non-invasive samples such as saliva and urine was studied for the molecular diagnosis of varicella by conventional polymerase chain reaction (PCR), real-time PCR and real-time loop-mediated isothermal amplification (real-time LAMP).

Results:

The results of the present study showed that saliva and urine samples can be used for outbreak investigation of varicella compared to varicella-zoster virus DNA in vesicular swab samples with reasonable sensitivity.

Conclusion:

Thus, molecular techniques may be useful in the early identification of the outbreak and timely isolation, and the treatment of cases can further prevent its spread.

KEYWORDS:

Loop-mediated isothermal amplification; polymerase chain reaction; varicella

PMID:
31424006
DOI:
10.4103/ijmm.IJMM_18_264
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