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Mol Nutr Food Res. 2019 Aug 18:e1900307. doi: 10.1002/mnfr.201900307. [Epub ahead of print]

Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction.

Wu C1,2, Pan LL3, Luo Y1,2, Niu W1,2, Fang X1,2, Liang W1,2, Li J1,2, Li H1,2, Pan X1,2, Yang G4, Chen W1,2, Zhang H1,2, Lakey JRT5, Agerberth B6, de Vos P7, Sun J1,2.

Author information

1
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.
2
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.
3
School of Medicine, Jiangnan University, Wuxi, 214122, P. R. China.
4
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, P. R. China.
5
Department of Surgery, University of California Irvine, Orange, CA, 92868, USA.
6
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital, 17177, Stockholm, Sweden.
7
Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, 9713 GZ, Groningen, The Netherlands.

Abstract

SCOPE:

This study aims to examine the protective effects of specific low-methoxyl pectin (LMP) on the development of type 1 diabetes (T1D).

METHODS AND RESULTS:

Female non-obese diabetic (NOD) mice are weaned onto either control or 5% LMP supplemented diets for up to 22 weeks of age. T1D incidence, gut barrier function, and pancreatic-gut immune responses are analyzed. LMP supplementation significantly dampened the onset of T1D in NOD mice. LMP supplementation induces caecal homeostasis, as indicated by the increasing SCFAs production, higher expression of tight junction proteins claudin 1, zonula occludens-2 in caecum. Furthermore, LMP-mediated caecal homeostasis impacts gut-pancreatic immunity, as evidenced by increased regulatory T cell population, modulated inflammatory cytokine expression, and suppressed NOD like receptor protein 3 (NLRP3) inflammasome activation in both caecum and pancreas.

CONCLUSION:

The data demonstrate that LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment, providing a scientific basis for using LMP as a novel functional supplementation to intervene T1D.

KEYWORDS:

autoimmune diabetes; caecal homeostasis; immunomodulation; low-methoxyl pectin

PMID:
31423661
DOI:
10.1002/mnfr.201900307

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