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Naunyn Schmiedebergs Arch Pharmacol. 2019 Aug 16. doi: 10.1007/s00210-019-01710-6. [Epub ahead of print]

Coptisine ameliorates renal injury in diabetic rats through the activation of Nrf2 signaling pathway.

Author information

1
Department of Endocrinology, Xijing Hospital, Air Force Medical University, 15 West Changle Road, Xi'an, Shaanxi, 710032, People's Republic of China.
2
Department of Geratology, Xi'an Ninth Hospital, Xi'an, Shaanxi, 710054, People's Republic of China.
3
Department of Clinical Medicine, School of Queen Mary, Nanchang University, Nanchang, Jiangxi, 330031, People's Republic of China.
4
Department of Neurology, Xi'an Electric Power Central Hospital, Xi'an, Shaanxi, 710032, People's Republic of China.
5
Department of Endocrinology, Xi'an Ninth Hospital, Xi'an, Shaanxi, 710054, People's Republic of China.
6
Department of Internal Medicine, 522nd Hospital of Chinese PLA, Luoyang, Henan, 471003, People's Republic of China.
7
Department of Endocrinology, Xijing Hospital, Air Force Medical University, 15 West Changle Road, Xi'an, Shaanxi, 710032, People's Republic of China. xiaomiao@fmmu.edu.cn.

Abstract

The present study has been designed and carried out to evaluate the potential of coptisine on diabetic nephropathy. Diabetes was induced in SD rats through one single intraperitoneal injection of streptozotocin (65 mg/kg) method, and then diabetic rats were orally administered with 25 mg/kg/day coptisine or 50 mg/kg/day coptisine for 8 weeks. Severe impairment of renal function in rats with diabetes was observed as indicated by increased urine protein excretion, kidney hypertrophy index, serum creatinine level, and blood urea nitrogen level. Oxidative stress damage was observed as indicated by increased levels of reactive oxygen species, malondialdehyde, and decreased levels of glutathione, superoxide dismutase, and catalase. However, these alterations in kidneys of rats with diabetes were alleviated by administration of coptisine. Furthermore, the expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its targeted antioxidative genes heme oxygenase 1 and NADPH quinone oxidoreductase 1 in the diabetic kidneys were significantly increased after coptisine treatment. These results suggested that coptisine ameliorated oxidative renal injury in diabetic rats, and the possible mechanisms for the renoprotective effects of coptisine may be related to activation of the Nrf2 signaling pathway.

KEYWORDS:

Coptisine; Diabetic nephropathy; Nrf2 signaling pathway; Rat

PMID:
31420722
DOI:
10.1007/s00210-019-01710-6

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