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Spectrochim Acta A Mol Biomol Spectrosc. 2019 Aug 7;224:117459. doi: 10.1016/j.saa.2019.117459. [Epub ahead of print]

Modulation of membrane properties by DNA in liposomes: A spectroscopic study.

Author information

1
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China. Electronic address: quhongjin@snnu.edu.cn.
2
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China. Electronic address: haochangchun@snnu.edu.cn.
3
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.

Abstract

Liposome mediated DNA transport possesses a number of preventing diseases in clinical trials, thus, the study of interaction between DNA and liposomes has become a hot research direction. In this paper, the adsorption behavior of DNA onto two representative lipids had been studied by the fluorescence spectrum measurement, Ultraviolet absorption spectrum and Langmuir-Blodgett technology. The results of fluorescence spectrum measurement indicated that the fluorescence liposomes were quenched statically by DNA at all three temperatures. Thermodynamic analysis displayed that the intermolecular forces between DNA and liposomes were van der Waals forces and Hydrogen bonding. The experimental results of Ultraviolet absorption spectrum and Langmuir-Blodgett technology further verified these mechanisms. This work provides useful theoretical basis for the development of novel DNA delivery materials.

KEYWORDS:

DNA; Fluorescence quenching; Lipid monolayers; Liposomes

PMID:
31419746
DOI:
10.1016/j.saa.2019.117459

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