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J Allergy Clin Immunol. 2019 Aug 12. pii: S0091-6749(19)31033-4. doi: 10.1016/j.jaci.2019.07.035. [Epub ahead of print]

Indoor bacterial microbiota and development of asthma by 10.5 years of age.

Author information

1
Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland; Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Mass. Electronic address: anne.karvonen@thl.fi.
2
Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland; Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland.
3
Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland.
4
Plant & Microbial Biology, University of California, Berkeley, Calif; California Department of Public Health, Environmental Health Laboratory Branch, Richmond, Calif.
5
Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Mass; Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care, Boston, Mass.
6
Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Mass.
7
Department of Pediatrics, Kuopio University Hospital, Kuopio, Finland.
8
Dr. von Hauner Children's Hospital, Ludwig-Maximilians-Universität, Munich, Germany; Member of the German Center for Lung Research, Giessen, Germany; Institute for Asthma and Allergy Prevention (IAP), Helmholtz Zentrum München, Munich, Germany.
9
Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland; Department of Public Health, University of Helsinki, Helsinki, Finland.

Abstract

BACKGROUND:

Early-life indoor bacterial exposure is associated with the risk of asthma, but the roles of specific bacterial genera are poorly understood.

OBJECTIVE:

We sought to determine whether individual bacterial genera in indoor microbiota predict the development of asthma.

METHODS:

Dust samples from living rooms were collected at 2 months of age. The dust microbiota was characterized by using Illumina MiSeq sequencing amplicons of the bacterial 16S ribosomal RNA gene. Children (n = 373) were followed up for ever asthma until the age of 10.5 years.

RESULTS:

Richness was inversely associated with asthma after adjustments (P = .03). The phylogenetic microbiota composition in asthmatics patients' homes was characteristically different from that in nonasthmatic subjects' homes (P = .02, weighted UniFrac, adjusted association, permutational multivariate analysis of variance, PERMANOVA-S). The first 2 axis scores of principal coordinate analysis of the weighted UniFrac distance matrix were inversely associated with asthma. Of 658 genera detected in the dust samples, the relative abundances of 41 genera correlated (r > |0.4|) with one of these axes. Lactococcus genus was a risk factor for asthma (adjusted odds ratio, 1.36 [95% CI, 1.13-1.63] per interquartile range change). The abundance of 12 bacterial genera (mostly from the Actinomycetales order) was associated with lower asthma risk (P < .10), although not independently of each other. The sum relative abundance of these 12 intercorrelated genera was significantly protective and explained the majority of the association of richness with less asthma.

CONCLUSION:

Our data confirm that phylogenetic differences in the microbiota of infants' homes are associated with subsequent asthma risk and suggest that communities of selected bacteria are more strongly linked to asthma protection than individual bacterial taxa or mere richness.

KEYWORDS:

Asthma development; Lactococcus species; children; diversity; environment

PMID:
31415782
DOI:
10.1016/j.jaci.2019.07.035

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