Send to

Choose Destination
J Gen Microbiol. 1988 Jan;134(1):29-36.

Alginate inhibition of the uptake of Pseudomonas aeruginosa by macrophages.

Author information

Department of Applied Biology, Brunel University, Uxbridge, Middlesex, UK.


Pseudomonas aeruginosa alginate was purified and characterized in terms of uronic acid, carbohydrate and protein content, as well as by infra-red spectroscopy and gel electrophoresis. Added exogenous bacterial alginate inhibited the uptake and degradation of both viable and non-viable radiolabelled non-mucoid P. aeruginosa by resident mouse peritoneal macrophages. Alginic acid (from seaweed) inhibited the same parameters to almost the same degree. Bacterial alginate also inhibited the uptake of fluorescent-labelled zymosan and latex particles. Starch, at equivalent viscosity to the alginate, inhibited the uptake and degradation of radiolabelled nonviable P. aeruginosa to a greater extent, but Dextran T500 had no effect. This suggests that the viscous nature of alginate exerts a non-specific inhibitory effect on the uptake and subsequent degradation of phagocytosible particles.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center