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Acta Naturae. 2019 Apr-Jun;11(2):92-97. doi: 10.32607/20758251-2019-11-2-92-97.

A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State.

Author information

1
The Federal Research Center Institute of Cytology and Genetics SB RAS, Lavrentyeva Ave. 10, Novosibirsk, 630090, Russia.
2
E.Meshalkin National medical research center Ministry of Healthcare of the Russian Federation, Rechkunovskaya Str. 15, Novosibirsk, 630055, Russia.
3
Novosibirsk State University, Pirogova Str. 2, Novosibirsk, 630090, Russia.
4
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentyeva Ave. 8, Novosibirsk, 630090, Russia.
5
AcademGene LLC, Lavrentyeva Ave. 6, Novosibirsk, 630090, Russia.
6
St. Laurent Institute, New Boston St., 317, 01801, Woburn, MA, USA.
7
A.P.Ershov Institute of Informatics Systems SB RAS, Lavrentyeva Ave. 6, Novosibirsk, 630090, Russia.

Abstract

Reprogramming of somatic cells to a pluripotent state is a complex, multistage process that is regulated by many factors. Among these factors, non-coding RNAs and microRNAs (miRNAs) have been intensively studied in recent years. MiRNAs play an important role in many processes, particularly in cell reprogramming. In this study, we investigated the reprogramming of rat fibroblasts with a deleted locus encoding a cluster comprising 14 miRNAs (from miR-743a to miR-465). The deletion of this locus was demonstrated to decrease significantly the efficiency of the cell reprogramming. In addition, the cells produced by the reprogramming differed from rat embryonic and induced pluripotent stem cells, which was an indication that reprogramming in these cells had not been completed. We suggest that this miRNA cluster or some of its members are involved in regulating the reprogramming of rat cells to a pluripotent state.

KEYWORDS:

CRISPR/Cas9; microRNA; pluripotent stem cells; reprogramming

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