Format

Send to

Choose Destination
J Clin Microbiol. 2019 Oct 23;57(11). pii: e00721-19. doi: 10.1128/JCM.00721-19. Print 2019 Nov.

Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini.

Author information

1
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA.
2
Johns Hopkins Hospital, Baltimore, Maryland, USA.
3
Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
4
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
5
Beckman Coulter, Inc., Sacramento, California, USA.
6
Johns Hopkins University School of Medicine, Baltimore, Maryland, USA kcarrol7@jhmi.edu law9067@med.cornell.edu.
7
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA kcarrol7@jhmi.edu law9067@med.cornell.edu.
8
Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, New York, USA.
#
Contributed equally

Abstract

The Staphylococcus intermedius group (SIG) is a collection of coagulase-positive staphylococci consisting of four distinct species, namely, Staphylococcus cornubiensis, Staphylococcus delphini, Staphylococcus intermedius, and Staphylococcus pseudintermedius SIG members are animal pathogens and rare causes of human infection. Accurate identification of S. pseudintermedius has important implications for interpretation of antimicrobial susceptibility testing data and may be important for other members of the group. Therefore, we sought to evaluate the performance of five commercially available identification platforms with 21 S. delphini isolates obtained from a variety of animal and geographic sources. Here, we show that automated biochemical platforms were unable to identify S. delphini to the species level, a function of its omission from their databases, but could identify isolates to the SIG level with various degrees of success. However, all automated systems misidentified at least one isolate as Staphylococcus aureus One matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system was able to identify S. delphini to the species level, suggesting that MALDI-TOF MS is the best option for distinguishing members of the SIG. With the exception of S. pseudintermedius, it is unclear if other SIG members should be routinely identified to the species level; however, as our understanding of their role in animal and human diseases increases, it may be necessary and important to do so.

KEYWORDS:

MALDI-TOF MS; Staphylococcus delphini; Staphylococcus intermedius group; automated biochemical platform; coagulase-positive staphylococci

PMID:
31413084
PMCID:
PMC6813025
[Available on 2020-04-23]
DOI:
10.1128/JCM.00721-19

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center