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Glycobiology. 2019 Nov 20;29(12):817-821. doi: 10.1093/glycob/cwz061.

Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots.

Author information

1
Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia.
2
Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2333 ZA Leiden, The Netherlands.
3
Laboratory of Prion Biology, Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati, Via Bonomea 265, 34136 Trieste, Italy and.
4
Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacica 1, 10000 Zagreb, Croatia.

Abstract

Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.

KEYWORDS:

IgG glycome; biomarker; dried blood spots; high-throughput; ultra-performance liquid chromatography

PMID:
31410450
DOI:
10.1093/glycob/cwz061

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