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Glycobiology. 2019 Nov 20;29(12):817-821. doi: 10.1093/glycob/cwz061.

Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots.

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Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia.
Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2333 ZA Leiden, The Netherlands.
Laboratory of Prion Biology, Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati, Via Bonomea 265, 34136 Trieste, Italy and.
Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacica 1, 10000 Zagreb, Croatia.


Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.


IgG glycome; biomarker; dried blood spots; high-throughput; ultra-performance liquid chromatography


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