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Biosci Biotechnol Biochem. 2019 Dec;83(12):2202-2212. doi: 10.1080/09168451.2019.1654359. Epub 2019 Aug 14.

Phenylethanoid glycosides from Paraboea martinii protect rat pheochromocytoma (PC12) cells from hydrogen peroxide-induced cell injury.

Gong X1, Xu Y2, Ren K3, Bai X4, Zhang C1, Li M1,3,5,6.

Author information

1
Department of Pharmacy, Baotou Medical College, Baotou, China.
2
Department of Emergency, The First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology, Baotou, China.
3
Intensive Care Unit, Inner Mongolia Autonomous region Hospital of traditional Chinese Medicine, Hohhot, China.
4
Department of Medicine, Xilinggol Vocational College, Inner Mongolia, China.
5
Guangxi key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
6
Pharmaceutical Laboratory, Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot, China.

Abstract

In this study, we isolated eight phenylethanoid glycosides from Paraboea martinii for the first time, and evaluated the mechanism underlying their neuroprotective effects against H2O2-induced injury in PC12 cells. The MTS method was utilized to screen the phenylethanoid glycosides for protective ability. Next, qRT-PCR and western blotting analysis were used to detect the transcription levels of HO-1 and GCLC, which are regulated by Nrf2. The inhibitor ZnPP was used to analyze the involvement of Nrf2 in HO-1 expression. Analyses showed that caleolarioside B, paraboside B, and paraboside II also upregulated the expression of HO-1, but showed no obvious effect on GCLC. Pretreatment with ZnPP significantly reduced the neuroprotective effects. Thus, phenylethanoid glycosides isolated from P. martinii protected PC12 cells from H2O2-induced damage by upregulating HO-1. The results provided evidence that P. martinii might be a potential therapeutic agent for the treatment of neurodegenerative diseases.

KEYWORDS:

HO-1; Paraboea martinii; neuroprotective effects; phenylethanoid glycosides; zinc protoporphyrin

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