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Stem Cell Res. 2019 Aug;39:101527. doi: 10.1016/j.scr.2019.101527. Epub 2019 Aug 7.

Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin: KCLi003-A.

Author information

1
Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK.
2
Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
3
Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.
4
Histology Laboratory, Wolfson Centre for Age-Related Diseases, School of Biomedical Sciences, King's College London, London, UK.
5
Skin Research Institute of Singapore, A*STAR, Singapore, Singapore.
6
Medical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia.
7
Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria.
8
Dermatology Services, Veteran Affairs Medical Center, University of California San Francisco, San Francisco, CA, USA.
9
Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK. Electronic address: dusko.ilic@kcl.ac.uk.

Abstract

We have generated an induced pluripotent stem cell (iPSC) line KCLi003-A (iOP101) from epidermal keratinocytes of a female donor, heterozygous for the loss-of-function mutation p.R501X in the filaggrin gene (FLG), using non-integrating Sendai virus vectors. Derivation and expansion of iPSCs were performed under xeno-free culture conditions. Characterization and validation of KCLi003-A line included molecular karyotyping, mutation screening using restriction enzyme digestion, next generation sequencing (NGS), while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay.

PMID:
31408836
DOI:
10.1016/j.scr.2019.101527
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