Format

Send to

Choose Destination
J Chem Neuroanat. 2019 Aug 10:101661. doi: 10.1016/j.jchemneu.2019.101661. [Epub ahead of print]

Monosynaptic tracing: a step-by-step protocol.

Author information

1
McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States.
2
McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States. Electronic address: wickersham@mit.edu.

Abstract

Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center