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Bioprocess Biosyst Eng. 2019 Aug 10. doi: 10.1007/s00449-019-02187-6. [Epub ahead of print]

Modulation of fungal biofilm physiology and secondary product formation based on physico-chemical surface properties.

Author information

1
Department of Microbial and Molecular Systems (M2S), KULeuven, 3001, Heverlee, Belgium.
2
Gembloux Agro-Bio tech, TERRA Research and Teaching Center, Microbial Processes and Interactions, University of Liège, Avenue de la Faculté 2B, 5030, Gembloux, Belgium.
3
Faculty of Chemical and Food Engineering, Institute of Technology of Cambodia, Phnom Penh, 12156, Cambodia.
4
Soft-Matter Physics and Biophysics Section, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200 D, Box 2416, 3001, Heverlee, Belgium.
5
Department of Biology, Ecology, Evolution, Freshwater and Oceanic Science Unit of Research FOCUS & Centre of Aid for Research and Teaching in Microscopy (CAREM), University of Liege, Campus du Sart-Tilman, Allée de six Août 15, 4000, Liège 1, Belgium.
6
Department of Food Technology, Gembloux Agro-Bio Tech University of Liège, Avenue de la Faculté 2B, 5030, Gembloux, Belgium.
7
Gembloux Agro-Bio tech, TERRA Research and Teaching Center, Microbial Processes and Interactions, University of Liège, Avenue de la Faculté 2B, 5030, Gembloux, Belgium. f.delvigne@uliege.be.

Abstract

Relative to the amount of knowledge concerning bacterial biofilms, little is known about the impact of physico-chemical properties of support material on fungal biofilm adhesion and physiology. In the field of industrial fermentation, large-scale production of low-cost fungal secondary product is a challenging area of research. In the present work, the effect of physico-chemical surface properties of five different materials (Teflon, glass, Viton™ rubber, silicon rubber, and stainless steel) on the production of class II hydrophobins (HFBI and HFBII) from Trichoderma reesei (HFB2a-2) and Trichoderma harzianum) was evaluated. Two culture systems (shake flask and drip flow reactor (DFR)) were used in this study to promote biomass growth and the production of hydrophobins. Furthermore, the effect of physico-chemical surface properties (hydrophobicity, surface energy) and surface texture (roughness) of support material on the initial colonization and attachment of the fungal biofilm was evaluated. Maximum biofilm productivity was obtained using Viton™ rubber for T. reesei and Viton™ rubber and stainless steel as support materials for T. harzianum. Scanning electron microscope (SEM) revealed that fungal biofilm adhesion was higher on the rough hydrophobic Viton rubber surface as compared to the smooth hydrophobic Teflon surface. Initial colonization initiated because of surface irregularities and holes in the material as hyphal filaments. Moreover, compared to traditional submerged fermentation, a significant increase in biofilm productivity for both strains (T. reesei, T. harzianum) in all five materials was obtained.

KEYWORDS:

Biofilm reactor; Hydrophobin; Solid-state physiology; Surface colonization; Trichoderma

PMID:
31401655
DOI:
10.1007/s00449-019-02187-6

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