Format

Send to

Choose Destination
Biosens Bioelectron. 2019 Oct 1;142:111567. doi: 10.1016/j.bios.2019.111567. Epub 2019 Aug 3.

Dual-readout immunosensor constructed based on brilliant photoelectrochemical and photothermal effect of polymer dots for sensitive detection of sialic acid.

Author information

1
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian, 350108, China.
2
Fujian Provincial Maternity and Children Hospital, Fuzhou, Fujian, 350108, China.
3
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian, 350108, China; Fujian Provincial Maternity and Children Hospital, Fuzhou, Fujian, 350108, China. Electronic address: dhong@fjnu.edu.cn.
4
Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Normal University, Fuzhou, Fujian, 350108, China.

Abstract

A delicate dual-readout immunosensor based on tetraphenylporphyrin-polymer dots (TPP-Pdots) with brilliant photoelectrochemical and photothermal performance was first successfully fabricated for the ultrasensitive detection of sialic acid (SA). Herein, TPP-Pdots with good biocompatibility, extraordinary light-harvesting ability and excellent photothermal conversion efficiency was used to capture SA antibody as dual-functional bioprobe for generating photocurrent and temperature signal. Furthermore, the large surface and morphology-mediated of rutile-TiO2 (R-TiO2) was beneficial to load amounts of TPP-Pdots for improved PEC signal and photothermal signal. Importantly, the temperature readout resulted from the variation of target concentration could be easily obtained by a universal thermometer which was time-saving and cost-saving. Under the optimized experimental conditions, the photocurrent densities and temperature changes proportionally increased with the increasing of SA concentrations from 3.5 × 10-5 ng/mL to 35 ng/mL (R = 0.996). Impressively, the dual-readout approach proposed here not only featured with good accuracy and high sensitivity for SA detection, but also paved the way for the development of a dual-readout immunoassay based on PEC biosensor.

KEYWORDS:

Dual-readout; Immunosensor; Photoelectrochemical; Photothermal; Salic acid; TPP-Pdots

PMID:
31400731
DOI:
10.1016/j.bios.2019.111567
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center