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Toxicol Lett. 2019 Aug 7. pii: S0378-4274(19)30219-X. doi: 10.1016/j.toxlet.2019.08.004. [Epub ahead of print]

An inter-laboratory in vitro assessment of cigarettes and next generation nicotine delivery products.

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Japan Tobacco Inc., R&D Group, Yokohama, Japan.
British American Tobacco, R&D, Southampton, UK. Electronic address:
British American Tobacco, R&D, Southampton, UK.


In vitro testing can facilitate the rapid assessment of next generation nicotine delivery products (NGPs) with comparisons to combustible tobacco products. In vitro assays for cytotoxicity and oxidative stress were employed at BAT (UK) and JT (Japan) to test total particulate matter (TPM) of a scientific reference cigarette and aerosol collected mass (ACM) of a commercially available E-cigarette and two tobacco heating products (THP). 3R4F TPMs were generated using the Health Canada intense (HCI) regimen, a modified regime (mHCI) for the THP ACMs and the CORESTA recommended method no. 81 for the E-cigarette ACM. Human lung cells were exposed to the test product TPM/ACMs at concentrations between 0-200 µg/ml followed by the employment of commercially available assays for endpoint analysis that included reactive oxygen species (ROS) generation, the glutathione ratio (GSH:GSSG), activation of the antioxidant response elements (ARE) and cellular viability. TPM/ACM nicotine concentrations were quantified using a UPLC-PDA technique. At both laboratories the 3R4F TPM induced significant and dose-dependent responses in all in vitro assays, whereas no significant responses could be measured for the NGP ACMs. In conclusion, both laboratories obtained comparable results across all endpoints therefore demonstrating the utility of the in vitro techniques combined with standardised test products to support the assessment of NGPs.


Smoke; cigarette; e-cigarette; epithelial; in vitro; next generation nicotine delivery products; nicotine; oxidative stress; tobacco heating product

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