Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia

Bianca Brawek; Olga Garaschuk

doi:10.1007/978-1-4939-9658-2_17

Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia

Methods Mol Biol. 2019:2034:231-241. doi: 10.1007/978-1-4939-9658-2_17.

Authors

Bianca Brawek¹, Olga Garaschuk²

Affiliations

¹ Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany.
² Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany. olga.garaschuk@uni-tuebingen.de.

PMID: 31392689
DOI: 10.1007/978-1-4939-9658-2_17

Abstract

Similar to many nonexcitable cells, microglia utilize intracellular Ca²⁺ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of the intracellular Ca²⁺ dynamics in microglia have been challenging due to technical reasons. Here, we describe an approach utilizing a single-cell electroporation technique to facilitate the study of microglial Ca²⁺ signaling in the living brain.

Keywords: 2-photon microscopy; Electroporation; In vivo calcium imaging; Microglia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytes* / cytology
Astrocytes* / metabolism
Calcium / metabolism*
Calcium Signaling*
Electroporation*
Mice
Microglia* / cytology
Microglia* / metabolism
Neurons* / cytology
Neurons* / metabolism

Substances

Calcium