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J Vasc Res. 2019 Aug 7:1-13. doi: 10.1159/000501614. [Epub ahead of print]

Comparison of Ca2+ Handling for the Regulation of Vasoconstriction between Rat Coronary and Renal Arteries.

Author information

1
Guangdong Provincial Key Laboratory of Clinical Pharmacology, Department of Cardiology, Guangdong Cardiovascular Institute, Guangzhou, China.
2
Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
3
Stomatology Center of Shenzhen People's Hospital, Second Affiliated Hospital of Jinan University, Shenzhen, China.
4
Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Zhuhai, China.
5
Guangdong Provincial Key Laboratory of Clinical Pharmacology, Department of Cardiology, Guangdong Cardiovascular Institute, Guangzhou, China, chunyudeng@126.com.
6
Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China, chunyudeng@126.com.

Abstract

BACKGROUND:

Ca2+ plays an important role in the regulation of vasoconstriction. Ca2+ signaling is regulated by a number of Ca2+-handling proteins. However, whether differences in Ca2+ handling affect the regulation of vasoconstriction in different arteries remains elusive.

OBJECTIVE:

To determine whether differences in Ca2+ handling affect the response to vasoconstrictors in different arteries.

METHODS:

Arterial ring contraction was measured using a Multi Myograph System. Vascular smooth muscle cells (VSMCs) were digested with type 2 collagenase in DMEM, then intracellular calcium concentration was measured with the Ca2+ probe fluo-4/AM in the isolated cells. Calcium-related proteins were assayed by Western blotting.

RESULTS:

Phenylephrine did not induce -coronary arterial contraction. There were differences in -5-hydroxytryptamine, 9,11-dideoxy-11a,9a-epoxymethano-prostaglandin F2a, and endothelin 1-induced vasoconstriction in different solutions between coronary and renal arteries. Vasoconstrictions in the presence of Bay K8644 were stronger in coronary than in renal arteries. Store-operated calcium (SOC) channels could mediate Ca2+ influx in VSMCs of both groups. SOC channels did not participate in the contraction of coronary arteries. In addition, there were significant differences in the expressions of receptors and ion channels between the two groups.

CONCLUSIONS:

Ca2+ handling contributed to the different responses to vasoconstrictors between coronary and renal arteries.

KEYWORDS:

Coronary artery; L-type calcium channel; Renal artery; Store-operated calcium channel

PMID:
31390638
DOI:
10.1159/000501614

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