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Biochimie. 1988 Mar;70(3):391-4.

Misaminoacylation and transamidation are required for protein biosynthesis in Lactobacillus bulgaricus.

Author information

1
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.

Abstract

Aminoacylation studies with Lactobacillus bulgaricus show that this organism possesses glutamyl-tRNA synthetase activity; however, glutamyl-tRNA synthetase activity cannot be demonstrated. Instead, Glu-tRNAGln, which is formed by glutamyl-tRNA synthetase, is amidated by a specific amidotransferase to Gln-tRNAGln. The amide donor in this reaction is glutamine. Thus, Gln-tRNAGln in this organism is not formed by direct glutaminylation of tRNAGln, but instead by a pathway which involves misaminoacylation and transamidation.

PMID:
3139057
DOI:
10.1016/0300-9084(88)90212-x
[Indexed for MEDLINE]

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