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Biotechniques. 2019 Sep;67(3):123-125. doi: 10.2144/btn-2019-0062. Epub 2019 Aug 6.

A modified gas-trapping method for high-throughput metabolic experiments in Drosophila melanogaster.

Author information

1
Charles Perkins Centre, The University of Sydney, Camperdown, NSW 2006, Australia.
2
School of Life & Environmental Sciences, The University of Sydney, Camperdown, NSW 2006, Australia.
3
School of Medical Sciences, The University of Sydney, Camperdown, NSW 2006, Australia.

Abstract

Metabolism is often studied in animal models, with the Drosophila melanogaster fruit fly model offering ease of genetic manipulation and high-throughput studies. Fly metabolism is typically studied using end-point assays that are simple but destructive, and do not provide information on the utilization of specific nutrients. To address these limitations, we adapted existing gas-trapping protocols to measure the oxidation of radiolabeled substrates (such as glucose) in multi-well plates. This protocol is cost effective, simple, and offers precise control over experimental diet and measurement time, thus being amenable to high-throughput studies. Furthermore, it is nondestructive, enabling time-course experiments and multiplexing with other parameters. Overall, this protocol is useful for merging fly genetics with metabolic studies to understand whole organism responses to different macronutrients.

KEYWORDS:

; carbon dioxide ; gas-trap ; metabolic labelling ; metabolism

PMID:
31385711
DOI:
10.2144/btn-2019-0062
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