Format

Send to

Choose Destination
Front Oncol. 2019 Jul 17;9:654. doi: 10.3389/fonc.2019.00654. eCollection 2019.

The Natural Compound Neobractatin Induces Cell Cycle Arrest by Regulating E2F1 and Gadd45α.

Zheng Z1,2, Wu M1,2, Zhang J1,2, Fu W1,2, Xu N3, Lao Y1,2, Lin L4, Xu H1,2.

Author information

1
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
2
Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery, Shanghai, China.
3
Key Lab in Health Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.
4
Perelman School of Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA, United States.

Abstract

The complexity and multi-target feature of natural compounds have made it difficult to elucidate their mechanism of action (MoA), which hindered the development of lead anticancer compounds to some extent. In this study, we applied RNA-Seq and GSEA transcriptome analysis to rapidly and efficiently evaluate the anticancer mechanisms of neobractatin (NBT), a caged prenylxanthone isolated from the Chinese herb Garcinia bracteata. We found that NBT exerted anti-proliferative effect on various cancer cells and caused both G1/S and G2/M arrest in synchronized cancer cells through its effects on the expression of E2F1 and GADD45α. The in vivo animal study further suggested that NBT could reduce tumor burden in HeLa xenograft model with no apparent toxicity. By demonstrating the biological effect of NBT, we provided evidences for further investigations of this novel natural compound with anticancer potential.

KEYWORDS:

E2F1; GADD45α; GSEA; RNA-Seq; cell cycle; neobractatin

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center