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Photodiagnosis Photodyn Ther. 2019 Jul 30. pii: S1572-1000(19)30318-7. doi: 10.1016/j.pdpdt.2019.07.026. [Epub ahead of print]

Anti-cancer, anti-biofilm, and anti-inflammatory properties of Hen's albumen: a photodynamic approach.

Author information

1
Department of Zoology, Scott Christian College, Nagercoil 629 003, Tamil Nadu, India.
2
Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Campus 6(th)Floor, Karaikudi 630004, Tamil Nadu, India.
3
Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India; Department of Zoology, Government College for Women, Kumbakonam 612 001, Tamil Nadu, India.
4
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
5
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: gkhaled@ksu.edu.sa.
6
Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Campus 6(th)Floor, Karaikudi 630004, Tamil Nadu, India. Electronic address: vaseeharanb@gmail.com.

Abstract

The albumen plays a major role in the protection of eggs against microorganisms. It contains an arsenal of natural antimicrobial molecules and antibacterial proteins, including the well-known ovotransferrin and lysozyme, which exert their activities against a range of bacteria. In the present study, the hen's albumen extract treated with the dried insect body of blister beetle M. pustulata was assessed for antibacterial, antibiofilm, anti-inflammatory and anti-proliferative activity. The zone of inhibition against Gram positive E. faecalis and S. aureus was 10.8 mm and 12.1 mm respectively at 100 µg mL-1. However, it was 13.6 mm and 15.3 mm for Gram negative P. aeruginosa and P. vulgaris respectively. The biofilm of tested bacteria was significantly inhibited at 100 µg mL-1. The hydrophobicity of bacterial biofilms was considerably condensed after treatment with the hen's albumen extracts at 100 µg mL-1. The anti-inflammatory activity of hen's albumen extracts was confirmed by the inhibition of cyclooxygenase (COX) enzyme to 84.91% at 100 μg mL-1 with the relative IC50 of 8.26 μg mL-1. The albumen extract effectively inhibited the viability (23.61%) of HepG2 hepatic cancer cells at 100 µg mL-1. The anti-proliferative activity of the albumen extracts was further revealed by the induction of HepG2 apoptotic cell morphology. This study concludes that the hen's albumen extract treated with M. pustulata is a natural therapeutic agent to treat biofilm associated clinical bacteria, inflammations and human hepatic cancer cells.

KEYWORDS:

CLSM; Light microscope; SDS-PAGE; biofilm; clinical bacteria; hepatic carcinoma

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