Format

Send to

Choose Destination
Science. 2019 Aug 2;365(6452):498-502. doi: 10.1126/science.aaw1720.

Isochorismate-derived biosynthesis of the plant stress hormone salicylic acid.

Author information

1
Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, D-37077 Goettingen, Germany.
2
Molecular Biology of Plant-Microbe Interactions Research Group, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, D-37077 Goettingen, Germany.
3
Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
4
Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, D-37077 Goettingen, Germany.
5
Department of Plant Cell Biology, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, D-37077 Goettingen, Germany.
6
Central Microscopy Facility of the Faculty of Biology and Psychology, University of Goettingen, D-37077 Goettingen, Germany.
7
Molecular Biology of Plant-Microbe Interactions Research Group, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, D-37077 Goettingen, Germany. ifeussn@uni-goettingen.de wiermer@uni-goettingen.de yuelin.zhang@ubc.ca.
8
Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. ifeussn@uni-goettingen.de wiermer@uni-goettingen.de yuelin.zhang@ubc.ca.
9
Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, D-37077 Goettingen, Germany. ifeussn@uni-goettingen.de wiermer@uni-goettingen.de yuelin.zhang@ubc.ca.
10
Department of Plant Biochemistry, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, D-37077 Goettingen, Germany.

Abstract

The phytohormone salicylic acid (SA) controls biotic and abiotic plant stress responses. Plastid-produced chorismate is a branch-point metabolite for SA biosynthesis. Most pathogen-induced SA derives from isochorismate, which is generated from chorismate by the catalytic activity of ISOCHORISMATE SYNTHASE1. Here, we ask how and in which cellular compartment isochorismate is converted to SA. We show that in Arabidopsis, the pathway downstream of isochorismate requires only two additional proteins: ENHANCED DISEASE SUSCEPTIBILITY5, which exports isochorismate from the plastid to the cytosol, and the cytosolic amidotransferase avrPphB SUSCEPTIBLE3 (PBS3). PBS3 catalyzes the conjugation of glutamate to isochorismate to produce isochorismate-9-glutamate, which spontaneously decomposes into SA and 2-hydroxy-acryloyl-N-glutamate. The minimal requirement of three compartmentalized proteins controlling unidirectional forward flux may protect the pathway against evolutionary forces and pathogen perturbations.

PMID:
31371615
DOI:
10.1126/science.aaw1720

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center