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J Cell Sci. 2019 Aug 19;132(16). pii: jcs233429. doi: 10.1242/jcs.233429.

The Chlamydomonas flagellar membrane glycoprotein FMG-1B is necessary for expression of force at the flagellar surface.

Author information

1
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
2
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
3
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA rds@dartmouth.edu.
4
The Marine Biological Laboratory, Woods Hole, MA 02543, USA.

Abstract

In addition to bend propagation for swimming, Chlamydomonas cells use their flagella to glide along a surface. When polystyrene microspheres are added to cells, they attach to and move along the flagellar surface, thus serving as a proxy for gliding that can be used to assay for the flagellar components required for gliding motility. Gliding and microsphere movement are dependent on intraflagellar transport (IFT). Circumstantial evidence suggests that mechanical coupling of the IFT force-transducing machinery to a substrate is mediated by the flagellar transmembrane glycoprotein FMG-1B. Here, we show that cells carrying an insertion in the 5'-UTR of the FMG-1B gene lack FMG-1B protein, yet assemble normal-length flagella despite the loss of the major protein component of the flagellar membrane. Transmission electron microscopy shows a complete loss of the glycocalyx normally observed on the flagellar surface, suggesting it is composed of the ectodomains of FMG-1B molecules. Microsphere movements and gliding motility are also greatly reduced in the 5'-UTR mutant. Together, these data provide the first rigorous demonstration that FMG-1B is necessary for the normal expression of force at the flagellar surface in ChlamydomonasThis article has an associated First Person interview with authors from the paper.

KEYWORDS:

Chlamydomonas; Cilia; FMG-1B; Flagella; Flagellar membrane; Gliding motility; Surface motility

PMID:
31371489
DOI:
10.1242/jcs.233429

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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