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Plant Cell Physiol. 2019 Sep 1;60(9):1974-1985. doi: 10.1093/pcp/pcz150.

Inhibition of Pre-mRNA Splicing Promotes Root Hair Development in Arabidopsis thaliana.

Author information

1
Department of Chemistry, Biology, and Environmental Science, Graduate School of Humanities and Sciences, Nara Women's University, Kitauoya-nishimachi, Nara, Japan.
2
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.
3
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan.
4
Synthetic Genomics Research Group, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, 1-7-22 Tsurumi-ku Suehirocho, Tsurumi-ku, Yokohama, Kanagawa, Japan.
5
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Japan.
6
Research Group of Biological Sciences, Division of Natural Sciences, Nara Women's University, Kitauoya-nishimachi, Nara, Japan.

Abstract

Root hairs protruding from epidermal cells increase the surface area for water absorption and nutrient uptake. Various environmental factors including light, oxygen concentration, carbon dioxide concentration, calcium and mycorrhizal associations promote root hair formation in Arabidopsis thaliana. Light regulates the expression of a large number of genes at the transcriptional and post-transcriptional levels; however, there is little information linking the light response to root hair development. In this study, we describe a novel mutant, light-sensitive root-hair development 1 (lrh1), that displays enhanced root hair development in response to light. Hypocotyl and root elongation was inhibited in the lrh1 mutant, which had a late flowering phenotype. We identified the gene encoding the p14 protein, a putative component of the splicing factor 3b complex essential for pre-mRNA splicing, as being responsible for the lrh1 phenotype. Indeed, regulation of alternative splicing was affected in lrh1 mutants and treatment with a splicing inhibitor mimicked the lrh1 phenotype. Genome-wide alterations in pre-mRNA splicing patterns including differential splicing events of light signaling- and circadian clock-related genes were found in lrh1 as well as a difference in transcriptional regulation of multiple genes including upregulation of essential genes for root hair development. These results suggest that pre-mRNA splicing is the key mechanism regulating root hair development in response to light signals.

KEYWORDS:

Arabidopsis thaliana ; Light regulation; Pre-mRNA splicing; Root hair development; Splicing factor; p14 protein

PMID:
31368506
DOI:
10.1093/pcp/pcz150

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