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Mol Nutr Food Res. 2019 Oct;63(20):e1900514. doi: 10.1002/mnfr.201900514. Epub 2019 Aug 12.

Prevention of Vascular Inflammation by Pterostilbene via Trimethylamine-N-Oxide Reduction and Mechanism of Microbiota Regulation.

Author information

1
Institute of Food Sciences and Technology, National Taiwan University, Taipei, 106, Taiwan.
2
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang, 438000, Hubei, China.
3
Sabinsa Corporation, 20 Lake Drive, East Windsor, NJ, 08520, USA.
4
Department of Food Science, Rutgers University, New Brunswick, NJ, 08901, USA.
5
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan.
6
Department of Health and Nutrition Biotechnology, Asia University, Taichung, 41354, Taiwan.

Abstract

SCOPE:

A gut-microbiota-dependent metabolite of L-carnitine, trimethylamine-N-oxide (TMAO), has been recently discovered as an independent and dose-dependent risk factor for cardiovascular disease (CVD). This study aims to investigate the effects of pterostilbene on reducing TMAO formation and on decreasing vascular inflammation in carnitine-feeding mice.

METHODS AND RESULTS:

C57BL/6 mice are treated with 1.3% carnitine in drinking water with or without pterostilbene supplementation. Using LC-MS/MS, the result shows that mice treated with 1.3% carnitine only significantly increased the plasma TMAO and pterostilbene supplementation group can reverse it. Additionally, pterostilbene decreases hepatic flavin monooxygenase 3 (FMO3) mRNA levels compared to carnitine only group. It appears that pterostilbene can alter host physiology and create an intestinal microenvironment favorable for certain gut microbiota. Gut microbiota analysis reveals that pterostilbene increases the abundance of Bacteroides. Further, pterostilbene decreases mRNA levels of vascular inflammatory markers tumor necrosis factor-α (TNF-α), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin).

CONCLUSION:

These data suggest that amelioration of carnitine-induced vascular inflammation after consumption of pterostilbene is partially mediated via modulation of gut microbiota composition and hepatic enzyme FMO3 gene expression.

PMID:
31368236
DOI:
10.1002/mnfr.201900514

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