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Ayu. 2018 Oct-Dec;39(4):220-225. doi: 10.4103/ayu.AYU_232_17.

Natural stains Zingiber officinale Roscoe (ginger) and Curcuma longa L. (turmeric) - A substitute to eosin.

Author information

1
Department of Oral Pathology and Microbiology, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India.

Abstract

Background:

Stained slides are of the utmost source of information in pathology. The routine stains utilize synthetic dyes which pose various health hazards which necessitate the importance of natural extracts as a histological stain.

Aims:

The aim of this study is to compare the efficacy of ginger and turmeric extracts as histological dyes in tissues keeping eosin as standard.

Objectives:

The objective of this study is to compare the staining potential, intensity, specificity, and shelf life of ginger and termuric extract dyes.

Materials and Methods:

Extracts of fresh rhizomes of Zingiber officinale Roscoe and Curcuma longa L. were obtained by dissolving them in alcohol, which was used to stain sections of 25 cases. Observer 1 compared the staining intensity, while observers 2 and 3 assessed preset parameters at regular intervals and subjected to Kappa statistics and Mann-Whitney U-test.

Results:

Z. officinale and C. longa solutions had pH 4-5 and temperature 24°C-27°C. Z. officinale showed better staining intensity and specificity to the cytoplasm and basic components of connective tissue as compared to C. longa. Crispness (P = 0.01) and background staining (P = 0.05) showed a significant difference. Shelf life of Z. officinale was better than C. longa.

Conclusion:

Z. officinale gave a better staining than C. longa and was found to be closest to eosin and is easily available, cost-effective and biodegradable.

KEYWORDS:

Curcuma longa; Zingiber officinale; eosin; natural stains

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