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Acta Neuropathol. 2019 Jul 30. doi: 10.1007/s00401-019-02049-1. [Epub ahead of print]

Detrimental and protective action of microglial extracellular vesicles on myelin lesions: astrocyte involvement in remyelination failure.

Author information

1
IRCCS Humanitas, via Manzoni 56, 20089, Rozzano, Italy.
2
CNR Institute of Neuroscience, via Vanvitelli 32, 20129, Milan, Italy.
3
Department of Neuroscience Rita Levi-Montalcini and Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Regione Gonzole 10, 10043, Orbassano, Italy.
4
Department of Excellence: Department of Pharmacological and Biomolecular Sciences (DiSFeB), University of Milan, via Balzaretti 9, 20133, Milan, Italy.
5
IRCCS Fondazione Don Carlo Gnocchi, via Capecelatro 66, 20148, Milan, Italy.
6
Department of Neurology, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI), University of Genoa, Largo Paolo Daneo 3, 16132, Genoa, Italy.
7
IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi 10, 16132, Genoa, Italy.
8
Department of Biotechnology and Translational Medicine, University of Milan, 20090, Segrate, Italy.
9
Humanitas University, via Manzoni 56, 20089, Rozzano, Italy.
10
Institute of Experimental Neurology (INSPE), San Raffaele Scientific Institute, Via Olgettina Milano 58, 20132, Milan, Italy.
11
CNR Institute of Neuroscience, via Vanvitelli 32, 20129, Milan, Italy. c.verderio@in.cnr.it.

Abstract

Microglia are highly plastic immune cells which exist in a continuum of activation states. By shaping the function of oligodendrocyte precursor cells (OPCs), the brain cells which differentiate to myelin-forming cells, microglia participate in both myelin injury and remyelination during multiple sclerosis. However, the mode(s) of action of microglia in supporting or inhibiting myelin repair is still largely unclear. Here, we analysed the effects of extracellular vesicles (EVs) produced in vitro by either pro-inflammatory or pro-regenerative microglia on OPCs at demyelinated lesions caused by lysolecithin injection in the mouse corpus callosum. Immunolabelling for myelin proteins and electron microscopy showed that EVs released by pro-inflammatory microglia blocked remyelination, whereas EVs produced by microglia co-cultured with immunosuppressive mesenchymal stem cells promoted OPC recruitment and myelin repair. The molecular mechanisms responsible for the harmful and beneficial EV actions were dissected in primary OPC cultures. By exposing OPCs, cultured either alone or with astrocytes, to inflammatory EVs, we observed a blockade of OPC maturation only in the presence of astrocytes, implicating these cells in remyelination failure. Biochemical fractionation revealed that astrocytes may be converted into harmful cells by the inflammatory EV cargo, as indicated by immunohistochemical and qPCR analyses, whereas surface lipid components of EVs promote OPC migration and/or differentiation, linking EV lipids to myelin repair. Although the mechanisms through which the lipid species enhance OPC maturation still remain to be fully defined, we provide the first demonstration that vesicular sphingosine 1 phosphate stimulates OPC migration, the first fundamental step in myelin repair. From this study, microglial EVs emerge as multimodal and multitarget signalling mediators able to influence both OPCs and astrocytes around myelin lesions, which may be exploited to develop novel approaches for myelin repair not only in multiple sclerosis, but also in neurological and neuropsychiatric diseases characterized by demyelination.

KEYWORDS:

Astrocytes; Extracellular vesicles; Mesenchymal stem cells; Microglia; Myelin lesion; S1P

PMID:
31363836
DOI:
10.1007/s00401-019-02049-1
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