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Plant J. 2019 Jul 29. doi: 10.1111/tpj.14485. [Epub ahead of print]

DELAY OF GERMINATION1-LIKE4 acts as an inducer of seed reserve accumulation.

Author information

1
Department of Horticulture, Oregon State University, Corvallis, OR, 97331, USA.
2
Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University, Wageningen, Netherlands.
3
Department of Biochemistry and Biophysics, School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, OR, 97331, USA.

Abstract

More than 70% of the global food supply depends on seeds. The major seed reserves, such as proteins, lipids and polysaccharides, are produced during seed maturation. Here we report that DELAY OF GERMINATION1 (DOG1)-LIKE4 (DOGL4) is a major inducer of reserve accumulation during seed maturation. The DOGL family proteins are plant-specific proteins of largely unknown biochemical function. DOGL4 shares only limited homology in amino acid sequence to DOG1, a major regulator of seed dormancy. DOGL4 was identified as one of the outstanding abscisic acid (ABA)-induced genes in our RNA sequencing analysis while DOG1 was not induced by ABA. Induction of DOGL4 caused expression of 70 seed maturation-specific genes even in germinating seeds, including the major seed reserves ALBUMIN, CRUCIFERIN and OLEOSIN. While DOG1 affects the expression of many seed maturation genes, the major seed reserve genes induced by DOGL4 are not altered by the dog1 mutation. Furthermore, the reduced dormancy and longevity phenotypes observed in the dog1 seeds were not observed in the dogl4 mutants, suggesting that these two genes have limited functional overlap. Taken together, these results suggest that DOGL4 is a central factor mediating reserve accumulation in seeds and that the two DOG1 family proteins have diverged over the course of evolution into independent regulators of seed maturation, with retaining some overlapping function.

KEYWORDS:

Abscisic acid; dormancy; hormone; seed development; seed maturation; storage proteins

PMID:
31359518
DOI:
10.1111/tpj.14485

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